Please tell me the formula and procedure for calculate the SOD activity by riboflavin method .please tell me the complete procedure with formula. I will be very thankful.
Hi Atul Srivastava Have you just added Riboflavin to the sample?
You also need to add NBT ( Nitroblue tetrazolium) to the sample.
This will inhibit the reaction and then you have to calculate the % inhibition.
Please follow the link and calculate accordingly. CHEERS!
http://www.worthington-biochem.com/sodbe/assay.html
you will find - in this file - all the details concerning SOD assay and several other parameters. good luck
Note this research and has a way to method to calculate SOD activity by riboflavin:
Article Some Antioxidant Enzymes in Workers Exposed to Pollutants
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