How to match the XRD of a compound for which there is no JCPDS file ? Is there any other characterization to claim the synthesis of desired compound?

I have synthesized LiMgF3 using the starting precursors LiF and MgF2 via solid state diffusion method. Now I am looking for some characterization to claim the desired product, as there is no...

09 February 2021 921 3 View

The objective is to perform clustering between normal people and hypertension people by using voice samples. How to proceed for this?

I have extracted MFCC, Chroma features from the dataset. Can anyone help me to tell what should be next step?

03 February 2021 5,536 6 View

How to calculate the % yield/conversion of Sucrose fatty acid ester by using GC, GCMS, HPLC and other?

Hi All, How to calculate the % yield/conversion of Sucrose fatty acid ester by using GC, GCMS, HPLC, and others? Please suggest.

19 November 2020 2,645 2 View

I am operating 2.5 MGD combined effluent treatment plant but the pH is increasing in the aeration tank and reaching 9.2 which is affecting microbial?

Any one can guide me that how can I increase MLSS concentration in aeration tank of Activated sludge process. The pH of influent coming from primary clarifier was 7.1 and after taking water in...

30 September 2020 8,846 7 View

Can 70 degree (or 60 degree) hot air kill coronavirus on the cotton clothes? If yes, then what should be the duration of hot air blow?

I want to sterilise some items covered safely in dry cotton cloth packings. For what duration, should I blow hot air so that the cloth is free from lethal Coronavirus?

18 May 2020 2,803 2 View

Irc calculation for dies alder reaction in gaussian software?

hoe we perform the organic or inorganic reaction in guassian program.what is the procedure of this calculation?

15 September 2019 493 2 View

Can i check absorbance values of Paclitaxel(PTX) using Elisa Microplate reader?

I use Elisa micro plate reader which supports 96-well plate. problem is my absorbance values are almost same for standards. I use Methanol as solvent with Paclitaxel at 230nm even i change...

05 August 2019 9,511 4 View

Can anyone help me with determining protein expression using quantitative Real time PCR?

I am working on a sperm specific protein, I want to determine its concentration in control and infertile. Can it be done using real-time PCR and how?

24 June 2019 9,292 9 View

Does Electro-spinning process reduce the size of nano-particles dispersed within the solutions?

I have worked on the incorporation of GO nano-particles in PVA nano-fibers. The layered structure of GO has been observed from its morphology using SEM, but I have seen a significant reduction in...

30 April 2019 6,131 6 View

What boundary condition i used in hot rolling in abaqus software ?

i am working on hot rolling of titanium in abaqus . but i am not able to move the plate into roller what boundary condition i should used . i have given the velocity to sheet , angular velocity to...

23 April 2019 7,725 2 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

How to define tie constraint between rigid body and another part in dynamic explicit model of Abaqus?

Hi dear researchers I connected a rigid body into the part of my structure by tie constraint and then I added displacement to rigid body, in static general step every thing is fine and these two...

28 February 2021 3,442 2 View