Can any one suggest me any article in terms of Winter Wheat LD50 value for EMS?

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Anupam Barh

Hi, Here by providing details of one article

Response of selected wheat genotypes to

ethylmethanesulphonate concentration, treatment

temperature and duration

V. N. Ndou, H. Shimelis*, A. Odindo and A. T. ModiScientific Research and Essays Vol. 8(4), pp. 189 -196, 25 January, 2013

Available online at http://www.academicjournals.org/SRE

DOI: 10.5897/SRE12.543

link is - http://www.academicjournals.org/article/article1380815573_Ndou%20et%20al.pdf

http://www.academicjournals.org/article/article1380815573_Ndou%20et%20al.pdf

Cheng Liu

I just read a phD thesis, which is written in Chinese, the conclusion of this thesis is that, 0. 3 -0.5 M EMS treat seed for 8 h is the best LD50, it depends on the wheat variety, when I studied in Japan some years agao, I learn from a Japanese, you should optimize the condition by yourself based on your own material, anyway, I would suggest make a gradient from 0.3 to 0.5 M to get the best LD50 you need.

Keiko M Tuttle

Each batch of EMS is slightly different than the one before, therefore a titration of kill curves should be performed to achieve an appropriate LD50 concentration for your particular wheat accession/cultivar/line. Not only will each EMS batch potency be different, but the response from different genotypes will also vary. Good luck =)

K.M.Sajid Ali

0.3 to 0.7 is being concluded in various articles with different temp as well as treatment duration but more specifically I am looking for spring wheat.,

@ Anupam Barh thanking you for your consideration, I have gone through the paper that you suggested, but the wheat verity which is being used in first link is summer wheat.

"Looking for more specific reference, with spring wheat "

@Keiko M Tuttle thanks for giving your opinion, but can you please elaborate what's your suggestion : is it that I have to use the same batch of EMS for every treatment, cant I use different batches of EMS belonging to same company. and can you please suggest me which solvent will be the best for preparing the stock and working solution.