There are a number of options that could suit you but it really depends on what you plan to do with the cleared tissue.
Chlorophyll is easily removed by an ascending ethanol series (50, 70, 90, 100%) leaving clear tissue, this will leave fragile samples and will not be quite as complete as chloral hydrate.
Commercial alternatives are available, such as histo-clear which has excellent tissue clearing properties or Visikol or ClearSee all of which are designed to replace choral hydrate or xylene clearing.
Alternatively there are a number of other compounds which clear tissue such as chlorallactophenol, 2,2'-thiodiethanol or the classic clearing agent xylene. Which is best to use will require a bit of trial and error and also some research as to which is most suitable for your end application.
1. Alcohol is used to remove chlorophyll because it breaks the outer membrane of the leaf. It is not used to remove chlorophyll; it is used to make the leaf colourless.
2. Chlorophyll content was estimated by spectrophotometry by 80% acetone extraction. The leaves were incubated at room temperature in a 1.5-mL tube with 1 mL 80% acetone solution for at least 24 h then clarified by centrifugation for 5 min at 15,000g.
3. The dechlorophyllization (n-hexane: water partitioning, activated charcoal bleaching, and ChloroFiltr® decolorization). Activated charcoal bleaching and ChloroFiltr® decolorization effectively removed the chlorophyll.
4. The solution of acidified chloral hydrate–glycerol solution was prepared by dissolving 45 g chloral hydrate into a solution consisting of 25 mL 4.2% HCl (1:8, 38% HCl to H2O) and 10 mL glycerol as in standard methods for removal of cholophyll.
5. The solution Visikol™ (Polychlorinated alcohol mixture) allowing the solution to span cellular membranes and organelles and enabling penetration of the solution deep into tissues.