1. Kindly share formula to find out the catalase activity in the soil samples
2. I followed the procedure of volumetric titration method by using potassium permanganate.
Given below is the procedure followed by me:
Take 2 g of soil and add 40 ml of distilled water. Then add 5 ml of 30% hydrogen peroxide and shake it for 20 min. After that add 5 ml of 3 N sulphuric acid. Then filter it through whatsman filter paper. Collect 25 ml aliquot and titrate it with 0.001 M potassium permanganate. (Kuprevich, 1951 ; Johnson and Temple, 1964)