For red blood cell analysis have to dilute with RBC diluting fluid for image analysis. I would like to know the dielectric constant and viscosity of RBC diluting fluid (pure dilution solution only without dilute with blood sample) for my future work.
Dear Yesu,
The following are two RBC diluting fluids and their composition.
Based on the composition of both solution their viscosity and dielectric constant are about the same of water, 1 and 80, respectively.
1. RBC Diluting Fluid:
RBCs are about 5 millions/cu mm of blood. Counting this much number is highly impossible. Therefore, the blood sample is diluted with the help of RBC diluting fluid. It fixes and preserves RBC. It is isotonic to red cells.
Following two types of RBC diluting fluid are commonly used:
Formalin Citrate Diluting Fluid:
Composition:
i. Trisodium citrate – 3 gm.
ii. Formalin – 1 ml.
iii. Distill water – 99 ml.
This diluting fluid is cheap and commonly used.
Haym’s Diluting Fluid:
Composition:
a. Sodium chloride -0.5 gm
b. Sodium sulfate -2.5 gm
c. Mercuric chloride -0.25 gm
d. Distilled water – 100 ml.
The RBC diluting fluid prevents haemolysis and removes unwanted blood cells.
Hoping this will be helpful,
Rafik