We have fish scale protein extract treated at different reaction temperature and in need to perform amino acid composition analysis by HPLC/ GC-MS. We are seeking for the collaboration.
Background: I am developing an RdRp assay for Dengue virus-3 and need to express and isolate the NS5 protein's RdRp domain (~101 kDa). My expression vector includes a GST tag followed by a 6x His...
13 July 2024 9,950 3 View
AI has the potential to improve the management of sleep apnea by personalizing treatment, enhancing diagnostic accuracy, and advancing our understanding of the condition.
03 July 2024 9,393 2 View
How can i dilute my master stock of 1 micromolar DNA template to different concentration by serial dilution
17 May 2024 3,641 2 View
I am performing Cut & Tag experiment. I was wondering if there is any alternative/replacement for TAPS pH 8.5 in single tube Tagmentation and pA-Tn5 release ( DNA purification) step of Cut and...
02 April 2024 4,214 0 View
Most of the Antibody company (having pr. Ab conc. range 0.2 mg/ml - 0.5mg/ml) websites suggests dilution around 1:200, but it seems not staining or faintly staining. What is the hand on experience...
28 March 2024 259 3 View
Hey All, I am currently trying to simulate an FMCW Lidar in Ansys HFSS which I used for RADAR Simulation and I cannot find a way out to perform Lidar based simulation. If anybody knows how to do...
20 March 2024 2,749 2 View
Sometimes when I touch my colleague or metal bodies. i fell shock with some minor electric sound.
27 February 2024 1,191 3 View
I am growing small cell lung cancer suspension cells. How do I separate dead cells from live cells while splitting the cells after confluency in flask?
28 January 2024 8,818 6 View
I can do Rietveld refinement of single multiple phase data, but I am not able to do multiple data sequential Rietveld refinement. I am using Topas V7.
21 January 2024 2,083 0 View
I did FTIR of a reactive dye's powder. But I see that the transmittance of 3 peaks went opposite direction of normal peaks and eventually the transmittance percentage became more than 100%. What...
17 December 2023 7,093 2 View
A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...
11 August 2024 9,801 2 View
Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...
11 August 2024 8,141 3 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?
09 August 2024 7,718 0 View
Hello experts, Does anyone know any free software about retention index prediction ?
08 August 2024 7,403 2 View
I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...
08 August 2024 8,329 0 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.
07 August 2024 8,782 3 View
Dear QE-users, In the method where full MS positive mode and PRM mode are used, we always get an incorrect auxiliary gas reading (41 instead of 25). This only happens in this method; other...
06 August 2024 4,953 0 View
Are there any suggestions or insights you can provide to help address this problem
06 August 2024 1,443 2 View