We are conducting phase solubility study of artesunate with cyclodextrin. The concentration of artesunate obtianed by using UV spectroscopic method, but the results are inconsistant at each time we do the experiment. Can any one help?
Hi Ayman,
To get a glance on the structure of your compound it seems to be a peroxide. That might decompose in the presence of CD (or in the presence of any oxidable compound).
There is a second issue (for me): I cannot see too much chromophores. You mentioned the UV concentration determination. At which wavelength did you the measurements? At low wavelengths cyclodextrins may disturb the concentration determination, because they have some absorption.
It's challenging to obtain reliable and consistent solubility results, especially for poorly soluble compounds, as discussed in the following article which should help understanding and addressing the issues.
http://www.usp.org/sites/default/files/usp_pdf/EN/USPNF/revisions/determination-of-thermodynamic-solubility-of-active-pharmaceutical-ingredients-for-veterinary.pdf
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