Can any one help me to know the key marker staining for osteogenesis in MCT3T3 cells?

24 October 2017 1 7K Report

I have great hope and confident in this scientist community in particular about RG. Before starting any work even though we study or work on many techniques. Researchgate suggestions, comments and debates helps me lot to troubleshoot the problems.
Now i am in need to study about osteogenic effect natural compound on MCT3T cells.
Can any one help me to know the marker parameters and cell staining to pinpoint the compounds efficacy on osteogenesis process.

I would recommend to look for alkaline phosphatase, osteocalcin, Runx2 and Bsp expression. Further, you could do Von Kossa staining for the mineralized matrix. Please also see: Article Antagonizing Midkine Accelerates Fracture Healing in Mice by...

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