How do I find molecular formulas from a mass spectrum?

Can anyone suggest me a way to find the molecule formula from a mass spectrum. If you have a link or software means, please share it. Thanks in advance.

11 December 2014 3,175 7 View

Antibacterial activity loss during storage at 4 degrees C?

I have screened hundreds of actinomycetes strains with antibacterial activity against bacterial pathogens. The strains were stored at 4 deg C. After 4-6 months of storage I have lost the activity...

08 September 2013 2,002 7 View

Detection of diaminopimelic acid (DAP) using TLC?

Identification of cell wall type is an essential step for the characterization of actinomycetes. I have prepared the cell wall hydrolysate using 6N HCl and carried out the silica gel TLC with the...

08 September 2013 599 1 View

Storage of Bioactive Secondary metabolites?

I would like to know how to store the Crude and Purified metabolites. I have stored the crude methanolic extract of plant and microbial metabolites at -20 degree C and -80 d c. It was extracted,...

06 July 2013 8,488 21 View

Strain number information in IJSEM journal?

The type strain is CMU-PLA07T (=JCM 16380T, = BCC 38279T). Here CMU-PLA07 is Chiang Mai University and strain no, What is the meaning of T? What is the meaning of =JCM 16380T, = BCC 38279T? Can...

05 June 2013 1,655 2 View

For the purification of secondary metabolites which strategy is best to get a single molecule?

1. Crude - Column chromatography - TLC - RP.HPLC 2. Crude - TLC - Column chromatography - RP.HPLC 3. Crude - TLC - RP.HPLC 4. Crude - Column chromatography - Preparative HPLC Can anyone suggest...

03 April 2013 2,851 11 View

Can anyone suggest to me how to escape from fungal contamination in the actinomycetes laboratory?

I have problems in actinomycete culturing, maintaining and during incubation periods. I am using petri plates for the screening of actinomycetes. During the incubation periods in the incubator I...

02 March 2013 5,645 5 View

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02 March 2021 3,060 3 View

What do you know about biotechnology art?

What do you know about biotechnology art?

02 March 2021 1,653 3 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...

24 February 2021 9,839 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

How was your experience with Biomedical and Biotechnology Journal?

How was your publication experience with BBRJ in terms of peer review and reviewer comments. Did you find it fair and transparent ?

23 February 2021 3,810 6 View

I have a problem with kanamycin - why the kanamycin doesn't affect?

Hi everyone, I need to expression of GFP with pET28 plasmid in E. coli BL21(DE3) plysE. Transformed cells have kanamycin and chloramphenicol resistance. Normally, only transformed cells should...

22 February 2021 4,032 4 View