I have four isolate of Botrytis cinerea. I have cultured them on PDA and incubated at 20°C and 18/6 dark/light regime for sporulation, but 3 of the isolates have very poor sporulation after 12 days. I want the isolates to reach maximum sporulation.

  • Is it possible that the medium or incubation condition are not suitable?
  • What I can do to improve sporulation of the isolates?

I will be appreciate if any body Can tell me about best medium and incubation condition (temperature and light regime) for best sporulation of Botrytis cinerea isolates?

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