Effect of TE buffer on the immobilization kinetics of functionalized oligos to the substrate.
You probably won't get much binding due to the amine content of the buffer, there'll be just too many. Use an MES or PBS instead. Nucleic acids will be better at RT for this reaction.
Yes tris is likely to be a problem. Just desalt the sample into a more appropriate buffer. I would personally use a buffer of much higher pH than is achievable with MES or PBS, as the resulting schiff base is more stable in alkaline buffer. Carbonate can be used for example. You may also need to stabilize the bond by reduction.
The others are right. Do not use TRIS as suspension buffer for amine modified oligos as Tris contains amine groups and will quench the reaction.
Resuspend the oligos in MES buffer with appropiate salts eg. NaCl, KCl, MgCl etc
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