I want to induce inflammation in the animal model (using chemical) and want to measure the inflammatory mediators like NO, TNF-alpha, IL-1 beta, IL-6 or PGE2 in the blood serum. Is it possible to increase the level of these mediators in serum through the treatment with conventional chemical inducer like acetic acid, formalin, caraginan or LPS? If anyone have the idea please share........
Thanks in advance.
LPS and carrageenan are normally used to induce an inflammatory response, but a cheaper option will be heat inactivated gram negative bacteria. Upon treatment you should be able to detect the above mediators in blood serum using ELISAs.
Hi Carrageenan and LPS will induce inflammatory markers that can be detected by ELISA. You can also try treating with heat killed bacteria.
Thank you so much Dr. Edmund. The thing is can you share with me any published protocol about this, please?
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