Hello,

I have difficulty to understand your question. What do you mean by "acclimation time"? Do you mean the period of acclimation to experimental conditions prior to experiment initiation? In experiments, acclimation time is important in all species. Because all species needs a time span to adjust their physiology to new conditions, especially aquatic species. Aquatic media is not like terrestrial media. In terrestrial media, species have no difficlty to adjust their metabolism to different oxygen levels and various toxic substance. However, in aquatic media, speceis should acclimate to new oxygen levels, pH, ammonia, nitrite, hardness, ions ... Also, poikilotherm species, needs to acclimate to new temperature as well.

Agree with previous answer. Acclimation for what? What will you do then? If it's a physiological test acclimation period or time is very important!

Hello,

thanks for answering..I refer in particular to the adptation time in  routine respiratory oxygen consumption experiments. I am agree with you that is fundamental in all species. I found some papers describing the adaptation time in cichlid but nothing regarding echinoderms (sea urchin in particular)...

Acclimation time for respirometry experiments is important in tropical reef fish. There are numerous papers describing acclimation times of 18-24 hours, which also include a fasting period. I would search for papers using the keywords brett style respirometer to bring up classic fish metabolic scope papers and to pinpoint the appropriate methodology you should use.

Hi,

The time of acclimation would surely effect the metabolic activity of aquatic animals with reference to osmoregulation based on variable of physico-chemical parameters of water like salinity, temperature, alkalinity, hardness, DO etc.

Dr Rao