There are many articles published showing the use of GC-MS to quantify PHA. I am struggling and ended up damaging GC column. I have been advised by a reliable and experienced GC supplier that NONE of the GC column would elute PHA, in fact, it will be hard to remove PHA from the injector once injected. But there are many report demonstrating the use of GC for PHA quantification, I am bit confused, WHO is right?. HELP
A component analyzed in the GC, it must be gaseous! I am not an expert on GC, but I think that normally components with a boiling temperature
GC-MS of PHAs refers to the analysis of the monomers present in the PHA polymer. The polymer is hydrolyzed to release the monomers and the monomers are derivatized through transesterification pretreatments such as methanolysis and ethanolysis. The derivatized monomers are volatile and so can be separated in the gaseous phase in a GC, whereas the polymer or the polar monomers cannot.