I am testing a particular concentration of a drug on mesenchymal stem cells. I would want to check its ability to enhance the chondrogenic differentiation potential of MSCs. Is it right that chondrogenic differentiation be stopped at day 7 during this time to analyze the change in genes and protein or is it necessary to continue the differentiate the cells for 21 days to analyze the effect of drug . Please suggest .
Hi justin
MSCs or CPCs usually take 2-3 weeks differentiate into chondrocytes. so it is better to study cellular senescence after 12-14 days. you can get more information from below mentioned paper.
Chondrogenic Differentiation of Bone Marrow-Derived Mesenchymal Stem Cells: Tips and Tricks
Luis A. Solchaga, Kitsie J. Penick, and Jean F. Welter
Methods Mol Biol. 2011; 698: 253–278.
doi: 10.1007/978-1-60761-999-4_20
- Badges
- Science topic
- Similar topics
- Chemistry
- Pharmacology
- Pharmaceuticals
- Drugs