I want to calibrate the colloidal probe (pre-calibrated) with 3.5um of the tip radius (Atomic force microscopy performed is Dimension Icon, sofware is Nanoscope V). I do not know could I use the "No touch" calibration ? then Do I need to move the probe to the sapphire to determine the deflection sensitivity and Sync distance by Advance calibration ? I confuse when we should use "No touch", "Touch" and "Advanced" calibration ?
Thank you in advance your answer.
Depending on what you wanted to do with the colloidal probe, you will need to calibrate the normal and and lateral spring constant where the latter is needed to calculate friction coefficient. I used thermal tune method to calibrate the normal spring constant and Sader's method ( Rev. Sci. Instrum. 75, 1988–1996 (2004)) to calibrate the lateral spring constant.
You can perform thermal tune method directly from the Nanoscope software by ramping the AFM probe against atomically flat surface i.e. silicon wafer.
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