I am currently working on antioxidant activity of drought stress plants specifically Catalase and Ascorbate peroxidase and most of the papers that I've read they quantify unit of enzyme activity in "mg of protein". Could you suggest other formula in determining antioxidant activity without determining the protein content?
thank you
units of the enzyme per gr of plant tissue fresh weight (U/gr FW)
George Zervoudakis
could you please give me the exact formula for that or any references thank you
Dear Kat
The units of the enzyme activity are μmoles (of the converted substrate or of the product) per minute under the specified conditions of the particular enzyme assay.
You can measure the enzyme units either with a standard curve or with an appropriate extinction coefficient.
You must also measure the exact Fresh Weight of the homogenized plant tissue in order to calculate U/gr FW.
According to the above, there is no demand for measuring the total protein content in order to quantify U/mgr protein
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