I am looking for reporter mice in which I am study the T cell activation via calcium signalling on 2-photon microscopy. The experimental plan is to isolate and enrich the T cells from the Ca+ signalling reporter mice and adoptively transfer them into recipient mice. Ideally, all the non-C57B6/L mice having calcium reporter would be preferable.
Hi,
Mice transgenic for calcium sensor GCaMP6f are available from Jackson (https://www.jax.org/strain/028865).Some other strains with other calcium sensors have also been developed (e.g. TN-XXL [PMID: 22929788] or jRGECO1a [PMID: 30308007]). However, all of them are on B6 background. I am not aware of any mouse strain with calcium sensor on some other background.
Hey, yes this is the problem that I am facinhg at the moment since I have to transplant the reporter T cells into B6 background transgenic mice.
Do you by any chance know a TCR reporter mice is non-B6 background? Is Nur77-GFP mice a suitable reporter for studying TCR signaling?