Haroon Shaikh

5 Questions 5 Answers 0 Followers

Questions related from Haroon Shaikh

Calcium signalling reporter mice?

I am looking for reporter mice in which I am study the T cell activation via calcium signalling on 2-photon microscopy. The experimental plan is to isolate and enrich the T cells from the Ca+...

02 February 2019 2,575 2 View

Protocol for lymph node transplantation in mice?

I am searching for a protocol for the transplantation of lymph node between mice. For my experiments, I plan to transplant lymph nodes from conditional KO mice into complete KO of MHC2 molecule...

05 May 2018 9,217 0 View

Does Jurkat cells gets stimulated upon lentiviral transductions?

I have performed Lentiviral transduction in Jurkat cells with a plasmid encoding the mEOS fused with the PH domain of Akt. My experimental plan is to visualize T cell synapse by stimulating the...

08 August 2016 7,617 4 View

Can anyone recommend a protocol for Jurkat T-cells stimulation with soluble anti-CD3 and anti-CD28 antibodies?

I am looking for a protocol for the stimulation of Jurkat T cells with soluble anti-CD3 and ati-CD28 antibodies. I am working on the T-cells stimulation and effects of on kinectics. In my protocol...

07 July 2015 6,467 4 View

Promega Wizard DNA Clean-Up System 'RESIN' - any thoughts?

Can I use Wizard Midiprep DNA purification Resin 7M Guanidine HCl (Cat #A770A) as an alternate to Wizard® DNA Clean-Up Resin (Cat# A731A) while using Promega Wizard DNA Clean-Up System for...

02 February 2014 6,514 2 View