Any one familiar with SOFC/SOEC simulations using Ansys Fluent ?

23 August 2020 8,231 3 View

Adjust annealing temperature or additives to enhance specificity of PCR?

Dear All, I have a very basic question about the PCR/RT-PCR/qPCR. The PCR primers and TaqMan probes are designed such that the primers/probe connot bind to the mismatched site but only...

15 June 2020 4,926 3 View

Crystallization of PLLA?

07 June 2020 2,395 6 View

How to define grain boundary hardness?

08 March 2020 3,388 11 View

Will the TaqMan probe length affect the fidelity of the qPCR assay?

Dear all, I am learning to design the qPCR assays recently. But I just don't know why my negative controls always give positive signal( I have changed my design twice). I checked the formation of...

07 October 2019 4,968 3 View

Effect of mRNA secondary structure on reverse transcription?

Dear Sir/ Madam, I am designing a primer for microRNA reverse transcription. But some microRNAs like hsp-mir-21-5p has a strong secondary structure. It forms a stable hairpin. Can anyone please...

08 September 2019 8,283 3 View

How to deal with the nonlinearity in electrical tomography?

The electrical tomography can be utilized to calculate the solid concentration or gas holdup in aqueous based multiphase flows. However, there usually exists a strong nonlinear relationship...

16 May 2019 197 1 View

How many base-pairs can be added to the pSin vector?

Recently I decide to add a 5.5 kb fragment to pSin vector will it affects the efficiency of virus packaging?

20 March 2019 9,786 2 View

How can I deal with the non-specific binding in XIST-RNA-FISH?

I'm trying to detect the XIST-RNA expression in Breast cancer cells using the Biotin and Digoxin probes. However, there are always non-specific bindings in my results. I have added RNase-inhibitor...

18 March 2019 3,761 1 View

Any UV light absorbing material at 193-248 nm?

Would like to coat the alloy surface with non-organic material to absorb the UV light at 193-248 nm. Please recommend some UV absorbing material. Thanks~

16 November 2018 1,466 1 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to upload pdf of my articles published?

How to upload pdf of my articles published?

03 March 2021 2,183 3 View

Would adding books published in Lambert Publishers increase my RG impact?

There are some books published in LAP. Shall I add it to the RG?

02 March 2021 2,753 1 View

Is Journal of Agricultural Research a predator?

it is published by Juniper

28 February 2021 354 4 View

How can I publish a manuscript in free or low cost journal with good H-Index?

Dear all I am going to publish my manuscript which is from my thesis in a good H-Index journal but I found them very expensive(I don't have financial support) based on the exchange currency of my...

28 February 2021 7,800 6 View

Which software is better to write systematic review paper?

We have seen some authors use some software to write review paper. As obvious, the study of all published papers concerning our studying field is not possible and these software can help us to...

27 February 2021 6,296 1 View

Can I put a figure that was drawn by Bio-Render in publication paper?

I want to publish paper in journal but I have figures which have drawn with bio-renders, can I put them in the paper or which other software I can use to draw them?

27 February 2021 8,179 1 View

How can I assign a velocity along one direction to CO2 molecules in a molecular dynamics simulation using GROMACS?

Dear all, I am trying to perform a MD simulation about the interaction between CO2 and a polymer. I create my model, solvate it and perform the NVT and NPT equilibration. Now I am not able to...

26 February 2021 10,014 3 View

Zno nano particles colour changed from white to grey in process of calcination any suggestions?

In the formation of Zno nano particles process, after drying at 120 degree C, my zno nano particles are completely white but when i calcine it for 450 degree C it converts into grey colour. i...

26 February 2021 2,435 4 View

CGA 300 Regulator for CO2 cylinder?

Has anyone ever tried to deliver CO2 with a CGA 300 regulator? I know that a CGA 320 regulator is standard for CO2. The issue is that I currently do not have any free CGA 320 regulators. I also...

25 February 2021 3,292 3 View