We are specifically using Polar columns, but due to some application we need to explore possibility of using RP columns for SFC. Is there any study on this.
Generally, SFC is normal phase chromatography. Water is used up to perhaps 5% in SFC due to solubility limits, so you are either running the SFC equivalent of non-aqueous reverse phase, or you are using a pressure gradient to change the mobile phase strength. Isocratic runs are always a possibility.
As most SFC systems can run with up to 50% modifier, the compound would need to be very non-polar.
Pressure gradients work because, unlike regular liquids, the SFC mobile phase is compressible so higher pressures make a denser mobile phase, which is better able to dissolve the sample, and allow faster elution of a compound.
Here's a fairly recent paper: Article Development of a C18 Supercritical Fluid Chromatography-Tand...
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