How to build a resilient supply chain for a cookies company?
I found study by Dubey et al. (2020) might help, Empirical investigation of data analytics capability and organizational flexibility as complements to supply chain resilience. Article Empirical investigation of data analytics capability and org...
Hi everyone, I've been working on differentiating human iPSCs to derive a pure and uniform culture of neural progenitor cells (NPCs). However, I'm encountering a significant issue during the...
01 July 2024 1,012 1 View
I have been working on optimizing acid hydrolysis of wood chips to obtain xylose and glucose for E. coli growth. With the conditions I have been using (15%,20%,25% v/w) of acid and time (45,60,...
09 February 2019 5,051 7 View
What is the impact of nozzle diameter on selecting layer thickness and raster width ? For 0.4 mm nozzle diameter, what are the achievable layer thickness and raster width.
12 October 2016 7,202 8 View
How can RBC aggregation assay done? Is it similar to Platlet Aggregometer!
16 July 2014 7,810 4 View
Can we use ZF in a sickle cell disease model?
30 June 2014 9,631 5 View
I am developing a predictive model for a water supply network that involves 20 influencing points. However, I only have historical data for 10 out of these 20 points. I would like to know how to...
10 August 2024 4,005 2 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
A Markov-like Model for Patient Progression" Markov Chain Monte Carlo (MCMC) Markov Chain Monte Carlo (MCMC) is a powerful computational technique used to draw samples from a probability...
05 August 2024 10,079 0 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
Read the journal article by Douglas M. Lambert, “The Eight Essential Supply Chain Management Processes,” Supply Chain Management Review, Vol. 8, No. 6 (2004), pp. 18-26
04 August 2024 9,919 4 View
How does the atmosphere affect the flow of matter and energy on Earth and flow of energy in the biosphere related to the flow of food through a food chain?
02 August 2024 9,644 0 View
Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...
31 July 2024 2,406 6 View
Can anyone with an MJ research / BioRad PCR machine from ~2010 or earlier tell me the external measurements (LxWxH) of the removable standard PCR alpha modules that can be removed from a PTC200 or...
30 July 2024 2,867 0 View
Hello all, I have been trying to follow a 2-stage PCR protocol used to amplify barcodes of a large yeast library, as per Nyugen et al. (2022) -...
30 July 2024 841 2 View