What can be the fast and reliable algorithm (without neural network) for face mask detection?

Have implement neural network based mask detection algorithm with different colors of masks and that is working with 99% accuracy (around 2 fails in 850 tests) but the CNN based algorithm is too...

06 June 2020 1,440 7 View

From where i can get open source Historical solar power generation data?

For prediction solar energy i need solar power generation data.

21 May 2020 8,609 4 View

How to get real and imaginary permittivity in output versus frequencies in COMSOL Multiphysics?

Dear Researchers, I am working on the electrical (dielectric) properties of epoxy-based nanocomposites using COMSOL Multiphysics. I have stuck at some point the software. I want to see real and...

20 May 2020 1,134 3 View

How can I analyze flouride through HPLC ?

How can I analyze flouride through HPLC ? is IC better over HPLC for fluoride analysis or ISE is evergreen? I am Curious to know any better analysis for ionic fluoride which is less time...

28 February 2020 5,129 4 View

What are the books related to machine learning using python programming for beginners?

I am new to python programming and machine learning. Are there any books that give good practice of programming using python? Also do suggest books for machine learning.

06 August 2019 577 5 View

Is there an ASTM standard for tensile tests on electrospun polymer films ?

I need to perform a tensile test on a 30 micron thick electrospun polymer film, and fabrication limits the maximum length of a specimen to 80 mm. Similar publications have used the ASTM D638...

12 February 2019 3,212 4 View

Where to obtain uniaxial,biaxial and shear test data for polyurethane for hyperelastic models in ANSYS ?

I want to analyse a component made from Polyurethane. The uniaxial and biaxial test data are required for the curve fitting of the stress strain curves of any hyperelastic model. Where can I find...

22 March 2018 780 3 View

Does anyone know if there are some companies providing good 12-welled polypropylene plates?

I need to test certain growth conditions and enzymatic activities.

27 February 2018 1,770 1 View

What could be an alternative to ethanol as a wash buffer for magnetic bead RNA extraction?

I want to avoid ethanol as it is known to hinder downstream amplification process. Also, I am in a process of developing an automated diagnosis of ZIKA and would want to avoid...

09 July 2017 4,716 3 View

How to get good sections of rat heart in cryostat without tissue falling off?

I wash rat heart (left ventricle) 2 times with PBS over an ice bath, dry it with paper towels and then embed it in OCT over cooling bath. Lately, while sectioning in cryostat tissue is falling...

23 May 2017 994 6 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

GST assay in microplate?

I'd like to know if anyone can provide me with a protocol for traditional GST enzymatic assay (with GSH and CDNB as substrates) in a 96 well microplate, for cell line and/or C.elegans...

11 February 2021 1,328 3 View

How do i prepare rat colon samples for ANTIOXIDANT assay?

I stored small pieces of rat colon in PBS at -20 and now I want to do SOD and MDA assay, I also need to do the Bradford assay to quantify the protein content in the samples. can anyone tell me...

07 January 2021 2,488 1 View

Requesting protocols (96 well plate) for superoxide radical scavenging activity and hydroxyl radical scavenging activity?

Hello everyone, I am currently working on the natural colour pigment, betalains. I am looking for 96- well plate adopted assay protocols for measuring superoxide and hydroxyl radical scavenging...

03 January 2021 2,414 2 View

Problem with GST-fusion protein elution- Can anyone help?

18 December 2020 4,559 6 View

What could be the cause of the Glutathione peak migration (LC Dionex, Kintetex EVO C18, water phase) ?

Hello, I need a help from those of you who work on Kinetex EVO C18 with water + 0.2% TFA mobile phase (isocratic). The conditions are constant (temperatures, flow, column, conditioning time)....

16 December 2020 4,582 3 View

IHC-P staining of the viral infection in the mouse pancreas?

Hi, I'm trying to stain a viral infection of the pancreas in paraffin sections of adult (3 month old) BALB/c mice. Prior to starting my histology analysis, I have titrated the organ to confirm...

10 December 2020 2,198 3 View

Number of cells for extacellular vesicles extraction?

hello! I want to extract vesicles from MDA MB 231 cell ligne, culture in serum free media for 48h, would you help me with th number of cells required to have the vesicles? thank you

03 December 2020 6,488 2 View

Infected plant protein lysate washing out purified GST-tagged protein on agarose resin?

Hi, I am trying to screen infected plant proteomes for targets of a secreted pathogen protein. I have purified the pathogen protein using a GST-tag on an agarose resin. I am using the Pierce...

23 November 2020 7,009 3 View

Why use STOP solution in ELISA?

At the last step for data analysis, we always use a STOP solution to stop the TMB oxidized by HRP. Why do we have to stop the reaction? Couldn't we measure the color wavelength of...

17 November 2020 5,177 3 View