Buffers for ROS assays?

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Why does soybean half-seed transformation use 1/10x B5 salts during co-cultivation?

I was curious as to why modern soybean half-seed protocols use 1/10x strength B5 salts during the co-cultivation step rather than full strength B5 salts? Also, are the B5 vitamins added at full...

07 May 2024 3,924 0 View

Can we have two or more blinding methods in a clinical trial?

When planning a randomized, double-blind trial with multiple arms, there are often challenges for blinding the study treatment/groups due to different formulations/packaging/dosage. E.g. Study...

17 December 2023 8,345 1 View

How do I design primers for my insert for golden gate cloning using a vector with AarI restriction sites?

I am trying to insert a gRNA into my lentiviral vector backbone (image is attached) at an AarI restriction site. I am unable to figure out how to design primers to add the restriction sites to my...

17 October 2023 4,468 0 View

What is the difference between variance and variation(from total variation)?

Can anyone provide a deeper insight between variance and variation(from variational calculus) from statistics and calculus perspective? Is there any relationship between these two terms? I want to...

15 October 2023 9,930 2 View

During the calculation of relative quantum yield, I come to about a term 'fully corrected fluorescence specta'. What does it mean? and how to perform?

Hello, Greetings of the day, I want to calculate the relative quantum yield w.r.t. quinine sulfate (QS). Before going to sample, I want to ensure my standard (QS) and tried to calculate the...

06 October 2023 6,586 2 View

Looking for solvents for subcutaneous peptide delivery?

I am working on peptide molecule for SC delivery for my thesis work. Which solvents (or co-solvent) shall I use other than PEG, PG, Glycerin to increase the solubility (drug loading) of peptide...

15 August 2023 4,834 4 View

What are the best and reliable gel filtration based-spin columns to purify DNA and RNA ?

I am trying to fluorescently label RNA and purifying the labeled RNA further. The protocol recommends using gel filtration based membrane in the spin columns.

10 August 2023 2,332 1 View

What is the difference between transient and steady state analysis in natural gas flow problem?

I am new to understanding the transient and steady state analysis of the natural gas pipeline grids. Are there any articles/textbooks that can be suggested which explain about the steady state and...

01 August 2023 9,052 1 View

Where can I buy an electrical stimulation electrode that targets cymba concha region of the ear?

Hello, I am building a prototype transcutaneous auricular vagus nerve stimulator as a part of my PhD. So far, I have only found t-vns.com that have a device with the electrode that targets cymba...

18 May 2023 2,658 0 View

Is an Autoregressive Process Causal?

What is a causal process? Can an AR process be called as a causal process since we are inferring a directed graphical model from the time series?

18 December 2022 6,924 3 View

Is perfusion required to stain brain tissue with every method?

I'm looking to immunostain some brain tissue collected previously. The animal was *not* perfused prior to tissue collection. Will the presence of blood interfere with all staining methods...

14 July 2024 177 4 View

Does anyone have this article in PDF and could they pass it on to me?

Paper: Wang, A.G., Luo, G.H., 1990. Quantitative relation between the reaction of hydroxylamine and superoxide anion radicals in plants. Plant Physiol. Commun. 6, 55–57.

19 June 2024 7,283 0 View

What to do when GST- tagged protein is getting precipitated during dialysis?

I purified my recombinant GST-tagged protein in Tris buffer at pH 7.6 (the protein’s PI is 7.11) using a 50 mM reduced glutathione concentration in the elution buffer. However, after dialysis to...

21 May 2024 9,951 2 View

What should I use to homogenize samples for measurements of Oxidative Stress?

I bought a lipid peroxidation kit from Sigma and I want to get kits to measure nitrates, superoxide dismutase, catalase, and glutathione. Each one of those kits comes with a different lysis...

07 May 2024 4,583 2 View

Incubation of antioxidants with viral inoculum experiment?

I would like to understand whether a specific frequency of light (in the visible spectrum) inactivates a viral species by oxidative process through ROS formation or by a conformational change of...

05 May 2024 2,109 0 View

Respected academician, I did staining of dormant and non-dormant seeds. Dont know how to explain these pics? please help ?

Peroxidase activity 📷📷 Dormant seed Non dormant seed

17 April 2024 7,384 2 View

Need a research topic related to GST?

I need a research topic related to recent trends in GST

16 April 2024 1,805 0 View

How to expression and purify full length TRAF2 protein from bacteria?

Hi, I am for the first time struggling with TRAF2 full length protein purification from BL21 or Gold but I fail to purify it although I tried GST, MBP and His tag it the N- terminal. Do any...

07 April 2024 1,090 0 View

How to calculate (tot) glutathione peroxidase activity (GPx)?

Hi, I have been analyzing tot glutathione peroxidase (GPx) activity in mussel cells using cumene hydroperoxide (microplates) and I am a bit unsure how to read/calculate the results. I run a...

03 April 2024 9,344 0 View

Is it possible an unspecific binding of streptavidine or peroxidase?

I have a question for the professionals. The essence of the problem: I fix the oligos on a plastic substrate, they play role of primers in solid-phase PCR. I carry out a one-step PCR with...

28 March 2024 9,110 2 View

Antonietta Siciliano

I usually use phosphate buffer, 50 mM: mix 50 mM Na2HPO4 solution and 50 mM KH2PO4 solution, pH 7.0.

Ayad Palani

No you can't each test needs it's own pH for analysis and sample homogenation

Karima Zitouni

I agree with Ayad, definately you can not use the same buffer. It all depends on which assay are you using. For example for GPX most assays recommend a basic (PH=8)