How to make a citrate buffer solution in which there is NaCl. Concentrations of citric acid and sodium citrate 5 mM and NaCl 150 mM.; thank you.
Hi, I would like to ask for a help from the Research Gate community regarding a statistical analysis. My case is analyzing the association between the concentration of HIV DNA prior to therapy...
25 April 2024 7,702 0 View
can we build point cloud from google street view? to step 3D models result
22 November 2023 5,763 0 View
Is there any explanation for strong, adequate or low value of it? Thank you
19 February 2023 2,520 4 View
Is a questionnaire that has been tested for validity and reliability, must be tested again when the questionnaire will be used? if there are already test results for a certain country's version,...
24 January 2023 3,339 4 View
Many irrigation performance indicators were used in irrigated area. in a case of rainfed area, new water pump installed from the river. what is the best of measurement of sustainable irrigation...
19 January 2023 8,628 5 View
Hi, I am writing a research article about HIV; mainly describing the use of biomarkers of infection to describe recent infection. The article that I am writing apparently gets to almost 5,000...
21 December 2022 8,568 3 View
I wanna ask about procedure in genetic transformation procedure. I am conducting the transformation using A. tumefaciens but have difficulties to control over growth of bacteria. I am usually...
14 August 2022 8,271 6 View
Dear colleagues, currently I am doing an inflammasome-related experiment using cells. I have a substance as a candidate for anti-inflammasome. However, I am a little bit confused about which step...
14 November 2019 2,692 3 View
We knew that inflammasome event induced by LPS and extracellular ATP could lead to the cell pyroptosis and death, but what about the effect of only ATP administration to the cell culture? Is it...
19 September 2019 3,895 2 View
Currently, I am studying about the inflammasome activity inhibition using a compound in cultured microglial cell lines. As the common procedure to analyze inflammasome activity is by measuring the...
08 October 2018 1,297 4 View
Hello everyone, A collaborator sent us his protocol in order to be able to use his immunohistochemistry kits. But one step contains PBS + 0.3% H2O2 + 0.1% sodium azide. Knowing the dangerousness...
23 July 2024 6,314 3 View
I need to make a stock solution for radioisotopic incubations of freshwater sediments to study dark primary productivity but I have some confusion as to how I calculate the concentration of 14C...
15 July 2024 5,898 0 View
During the release test of Amphotericin B using 1% Tween 80, i observed three peaks even in the standard. The first peak consistently yielded results around 500%, while the other peaks showed...
08 July 2024 1,330 0 View
I did some column tests in my lab where I needed to detect the DOC fractions using LC-OCD. For the abiotic columns I used sodium azide to restrict the biofilm formation. I did LC-OCD to detect the...
07 July 2024 468 0 View
Hello All, I've been facing difficulty in executing geometry optimization of a sodium polyanionic phosphate (Na4Fe3P4O15) crystal in CASTEP (please find the input and output files attached). When...
07 July 2024 8,626 2 View
Can this pH of 8 be due to the presence of other substances? Or do I have an error somewhere in the analysis? Thank you!
26 June 2024 9,093 4 View
I have been trying to grow Nicotiana benthamiana via tissue culture. I used ethanol and sodium hypochlorite with different concentrations and time duration for seeds sterilization and lastly...
18 June 2024 1,756 3 View
I want to replicate the third step, but there seems to be no reaction in an alkaline environment. However, sodium hydroxide is used in the literature. Should I switch to an acidic environment
05 June 2024 9,358 1 View
I'd like a powdered form of MCDB 131 Medium with sodium bicarbonate added, and I've noticed that not only is the powder form of this medium difficult to find with NaHCO3 already included, but the...
28 May 2024 1,631 2 View
Hello. I am here looking for help with the NO radical scavenging assay. I was working on setting up the assay with sodium nitroprusside and the Griess reagent as they do in most works. However, I...
27 May 2024 5,013 1 View