Hi, scientist community,

I am trying to improve the sample preparation for microscopy. The main problem is bubbles formation under coverslip when I put the coverslip on sample drop. Sample drop is in the center of spacer cavity. The spacer is an sticker for the microscope slide. Firstly I paste this sticker on the microscope slide, then I put the sample drop (I calculate the drop volume using cylinder volume equation based on sticker cavity properties) and finally I put the coverslip and I always see several bubbles. The problem is not only bubbles, also the mobility of this bubbles (they are not stable).

I would appreciate any suggestion to improve samples preparation using spacers.

Thanks in advance

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