hello
i use hepg2 and huh7 cells now, before, i buy huh7 from an institute and that was so good and i didn't have any problem with them. but after a problem in our -80 freezer, i buy new one but after 1 passage or 48 hours after defreeze, they go for some brownish aggregated mass , may be autolysis occur. i dont know why and what kind of contamination occurred? also, when i put them in incubator, after 24 hr , i saw some new root-like not filament of fungus, in my hepg2 cells.
i would be so appreciated if any one could help me.
what is that? and what should i do?
thank you
i attach the image of huh7 cells.
Hi Gilar,
I cannot identify the contaminant from the image. My recommendation would be to try to culture the media on a wide array of permissive culture media. If you get bacterial or fungal growth, isolates can be identified by MALDI-TOF mass spectrometry or genetic sequence analysis. Considering the amount of floating debris, if it is a contaminating organism, a direct inoculation protocol should be sufficient (see http://www.idexxbioresearch.com/sterility-testing-pricing).
Marcus
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