We have created MDA-MB-231 stable knockdowns vs controls for our gene of interest. To finish off the in vitro data and publish our work we wish to use a xenograft mouse model to look at metastasis and tumor growth differences etc in vivo.
Does anyone know a service provider that can do this work to complete our project?
Most services appear to use xenografts for drug testing using their own in house cell lines, where as we wish them to test our knockdown and control cell lines.
We've done MDA-MB-231 xenografts commercially for some years now (http://altogenlabs.com/xenograft-models/breast-cancer-xenograft/mda-mb-231-xenograft-model/) and you can find some example growth curves on our website. We can accommodate most research needs, and we can test custom knockout cell lines as well, just contact us directly with your experimental details and we'll gladly assist.
I suggest to look for an in-house or near by collaboration with a continuous dialog because you never know how your cells will behave in vivo. Are they bioluminescent/fluorescent? I think if you look for a service, they just do what your ask for and it may not answer your question. If you are interested in bone metastases, your collaborator should be able to do an intra-cardiac injection in mice.
We are located in Vancouver Canada but have this in vivo model running. We do both subcutaneous tumor model and experimental metastasis (iv) injection and have specific experience with the 231 line in these models. We do not typically do cardiac injection but are capable if this is desired. We are happy to work in collaboration - let me know if you would like to discuss further?
Hi Charles,
We can do it either as fee for service or collaboration. We can run orthotopic or s.c. and can image etc. please check out our papers in PNAS (Gjerdrum et al 2010) and Cancer Res (McCormack 2013). Based in Norway.
Hi
We have an well established,excellent Xenograft Facility, based in Ninewells Hospital, Dundee, Scotland. We do both subcutaneous and orthotopic (metasasis) xenograft work. We are a Breast Research Group, and have worked, with most breast cell lines, melanoma's, lung, oesophageal and colon cell lines invivo. Our papers are available here, online, please feel free to approach our group for advice or questions.
we are quite reputed group working in this area for quite long time. we would be happy to discuss more in detail
With regards
Mani
We are happy to help you!
www.pharmatest.com
Regards,
Dr. Johanna Tuomela
Thanks to all answers. This has been one of the most humbling experiences in being able to begin an international collaboration to finish off some much needed work. I thank you all!
If you need some assistance on this side of the pond our group at Vanderbilt University has extensive experience with MDA-MB-231 and a dozen or so of its variants and subclones.
We've done MDA-MB-231 xenografts commercially for some years now (http://altogenlabs.com/xenograft-models/breast-cancer-xenograft/mda-mb-231-xenograft-model/) and you can find some example growth curves on our website. We can accommodate most research needs, and we can test custom knockout cell lines as well, just contact us directly with your experimental details and we'll gladly assist.
That is great. Thanks Michael. This original project is now done - but I will certainly look you up for follow up projects - especially to generate pilot data.
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