06 April 2023 0 7K Report

Hi, I am planning to run ELISA to determine CB1 receptor concentration in Mice hippocampus. The manufacturer protocol mentions to only use ice cold PBS to homogenize the brain samples. Is it sufficient or i need to use RIPA buffer with protease inhibitor?

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Which ethical metrics is expected from an academic who is the owner or the creator of the measuring instrument?

Which ethical metrics is expected from an academic who is the owner or the creator of the instrument? The measurement tool will be applied to adults as an online form and a snowball sample will...

01 February 2021 9,188 3 View

Hi my name is syahida. may i know the accurate concentration of lpg gas?? is it 250 ppm consider as dangerous gas in household?

hi my name is syahida. may i know the accurate concentration of lpg gas?? is it 250 ppm consider as dangerous gas in household? can you guide me to do the project of lpg gas detector system...

23 January 2021 9,425 4 View

What the reliability method of filling missing time series data?

Hi Researchers, How to measure the reliability of the method for filling missing time series streamflow data such as ARIMA model and interpolation?

12 January 2021 2,725 7 View

How to standardize and set parameters in the Puller sutter P-97 to get accepted pipette shape and resistance for whole cell recordings on DRG?

We are working on a P-97 puller and we are using it to do whole cell recording in DRG. We have 2 types of pipettes (borosilicate glass ID: 1.1 OD: 1.5 and ID: 0.86 OD: 1.5 ) as it showed in the...

04 January 2021 2,112 6 View

How do I write in a more professional scientific way?

I am an undergraduate in university. I am new to scientific research and would like to know how to make my paper more professional. Thanks in advance.

04 January 2021 264 12 View

What is the difference between Introduction, Background, Literature Review and Conclusion for writing a Thesis Report?

I've recently started writing a thesis report based on my research paper. And I'm very confused about writing the chapter of the literature review because we already discusse everything in the...

03 January 2021 6,028 8 View

In the case of SEM (scanning electron microscopy) of Fasciola gigantica, some white spots/substances were found but what are the causes for these?

In the case of SEM (scanning electron microscopy) of Fasciola gigantica, some white spots/substances were found but what are the causes for these?

03 January 2021 8,525 3 View

How can the moment tensor inversion results can present good waveform fit (synthetic and observed waveform) from generalized Cut and Paste scheme?

Depending upon the distance of the seismic station from the epicentre, the quality of waveform data and the improvisation of the synthetic waveform on it is often a question. If the Pnl waveform...

31 December 2020 6,602 2 View

Does "response to item ratio" has any effect on "inter-item correlation"?

Suppose a survey was conducted with a lower sample size (less than 100) for factor analysis of the items (less than 20). Now would a larger sample size increase the inter-item correlation? What...

27 December 2020 1,722 3 View

How to calculate covariance of Expectation Maximization Clustering?

I am working with MatLab R2017a, I have to implement EM clustering. But I am stuck with initialization of covariance in it. I have to calculate covariance using the dataset which is comprised of...

25 December 2020 4,620 3 View

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What is the ideal efficiency for a qPCR?

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