SM Nageeb Hasan

3 Questions 2 Answers 0 Followers

Questions related from SM Nageeb Hasan

Has any one previously experienced standard curve depression in mouse Amyloid Beta 42 ELISA kit from Invitrogen?

I am running mouse amyloid beta 42 ELISA using the kit from Invitrogen. According the manufacturers guideline, the samples should be diluted so that the concentration of guanidine in final...

08 September 2023 7,815 2 View

Brain tissue homogenization protocol for ELISA?

Hi, I am planning to run ELISA to determine CB1 receptor concentration in Mice hippocampus. The manufacturer protocol mentions to only use ice cold PBS to homogenize the brain samples. Is it...

06 April 2023 6,904 0 View

Lysis buffer and Protease Inhibitor ratio during tissue homogenization for ELISA?

Hi All, I am going to run BDNF ELISA in mouse brain tissue (prefrontal cortex and hippocampus). Can any one suggest a good lysis buffer and in what ratio should the protease inhibitor be mixed...

23 October 2021 618 1 View