we are currently trying to develop beta-glucan staining of live C. albicans with biotinylated FcDectin1 (and biotinylated FcClec1 as negative control). there seems to be non-specific binding of FcClec1 to the hyphae (extremely bright fluorescence compared to FcDectin1 stained hyphae). I've tried different Tween20, BSA and/or NaCl combinations/concentrations to no avail. The Candida and staining reagents are prepared in RPMI medium with 10% FCS.

Also does anyone know of a way to block excess streptavidin binding sites? As I intend to use other soluble receptors together with the FcDectin1-bio.