Hello everyone, You know what is the blank for the FRAP Assay?, to calibrate to zero the FRAP solution, or What is the absorbance of FRAP solution?, (control absorbance, for example in DPPH is around 0.6).
Thank you for the answers
To calibrate to zero put a blank containing everything except for the analyte of interest.
Hello Manju Tembhre, thanks for the answer, but can you tell me what is the blank to read the absorbance only for the FRAP solution?, without the analyte, because I am working with a methodology that requires the absorbance of only the initial reagents (FRAP solution). Get my kind regards.
Hello Melissa, Better you take reading for I blank/ zero it using FRAP reagent solution.
Hello Mellisa, for any blank solution we generally replace the sample with (say) DH2O. or sometimes the main reagent, here FRAP reagent with DH2O. If your objective is only to get the absorbance of FRAP reagent then I think we can use the DH2O alone. But what I understand from your question is... you are somehow correlating DPPH assay with FRAP assay which is not correct. For DPPH, we calculate the % inhibition and therefore the concept of control absorbance. Here in case of FRAP assay, if you are following the Benzie and Strain method (1996) we have to draw a standard curve for FeSO4 just like others. FRAP assays estimates our sample's Ferric Reducing capacity and therefore we can't calculate % inhibition unlike DPPH or ABTS assays. Once you get the standard curve data, you can correlate to your sample data.
Hope this will help you.
- Badges
- Science topic
- Similar topics
- Microbiology
- Assays