Hi Archana,

for unkown gene you should first begin with blast from NCBI (https://blast.ncbi.nlm.nih.gov/Blast.cgi) and see what is closed to your gene and species.

fred

Hello Archana,

You can perform blast. You can also do pfam search to know motifs and domain search using CDD ncbi (https://www.ncbi.nlm.nih.gov/Structure/cdd/wrpsb.cgi).

Best regards,

Rajesh

Hi! You can start with Blast from NCBI, using the tool Nucleotide to Nucleotide: https://blast.ncbi.nlm.nih.gov/Blast.cgi?PROGRAM=blastn&PAGE_TYPE=BlastSearch&LINK_LOC=blasthome

Or even using Nucleotide to protein (Blastx) https://blast.ncbi.nlm.nih.gov/Blast.cgi?PROGRAM=blastx&PAGE_TYPE=BlastSearch&LINK_LOC=blasthome

And you can also select one specific group of organism (you can type fungi) that you want to look for related or the same gene in closely related species.

After, if you identify some specific gene you can also use Uniprot, to search for the protein that is codified by that gene and look for the functions of that protein.

I hope this can help you!

Hi, you can use BioEdit and DNAsp softwares