Hello Everyone,
Our lab studies multiple sclerosis, and we're working on identifying targets for remyelination therapies using an animal model of demyelination. Focal demyelinated lesions are produced by injecting 1% lysolecithin (L-alpha-lysophosphatidylcholine from egg yolk) into the corpus callosum of mouse brains. To evaluate one of our drugs, we have chosen to co-inject either drug or vehicle alongside lysolecithin. We see differences, but the obvious question is whether the drug simply antagonizes lysolecithin or actually promotes restorative changes. I could answer this by performing seperate injections of lysolecithin followed by drug, but this increases risks for my mice and will require more animals moving forward. Anyone have a suggestion for an assay to assess whether my drug binds to lysolecithin?
Have you an idea about in vitro binding assays of your drug. Is there any study done with radiolabeled drug binding to lysolecithin. This could help about the potential of your drug.
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