Hi all,
I would like to generate amyloid beta oligomers for treatment on glial cells to assess cytokine production. I have come across a number of protocols but have been unsuccessful in obtaining any sort of consistent cytokine production.
I came across the paper below which mentions that post-HFIP evaporation, the peptide should be a clear film. White or chunky material left-over after evaporation is indicative of poor peptide quality. I have been using human abeta 1-42 from Anaspec and after evaporation my product is always a white film.
Can anyone comment on the color of the film after evaporation? Is the white film a problem? Should I look into getting abeta from a different vendor?
Any abeta oligomer protocols that people have used with success in glial cultures would be appreciated. Article Preparing Synthetic A?? in Different Aggregation States
Thanks!
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