I would like to apply a stain that will really make the diagonal band in the pigeon brain "pop-out". I can identify it in cresyl violet-stained sections, but sometimes it is quite difficult to make out boundaries. I'm thinking luxol fast blue (stain for myelin) may be a good. Suggestions, please?
Dear VIncent, I have used toluidine blue to contrast anatomical structures, this kind of stain is mainly used in electron microscopy but I started using it in conventional histology and also in whole brain in order to contrast the brain surface and it's an excellent colorant because it let you to diferentiate between structures. Good luck!!
From my experience in rodent brains, cresyl violet is sufficient to visualize neurons in the diagonal band and to delimit it. Of course, myelin staining could be a good alternative, but I'm not sure that luxol fast blue is the best one.
I have work on guinea fowl, rock pigeon and domestic Fowl. Cresyl fast violet is good. i will suggest luxol fast blue to you. the stain will mark out the diagonal band in the pigeon according to my understanding.
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