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Questions related from Vincent J. Coppola
Does tissue need to be perfused for congo red staining, or can is be done on non-perfused (i.e., bloody tissue)? Also, is 10% formaldehyde in PBS an appropriate fixative?
03 March 2019 6,234 1 View
I would like to apply a stain that will really make the diagonal band in the pigeon brain "pop-out". I can identify it in cresyl violet-stained sections, but sometimes it is quite difficult to...
04 April 2015 1,472 3 View
I have recently begun working with BrdU to label proliferating cells in the VZ of pigeons (thus, this is a 24 hr survival) -- the quality of the IHC has been less than impressive, but that's...
03 March 2015 7,663 1 View
Everything looked good until I exposed the skull and found that the brain was bloody. I planned to do DAB-IHC for BrdU in the ventricular zone. Is there anything I can do pre- or post-cutting to...
03 March 2015 1,336 7 View
Does anyone know if a Corning 24-well tissue culture plate can be heated to 37-degrees C (98.6-degrees F) without any issues? The last thing I want is the plate to melt, or some other catastrophic...
02 February 2015 8,014 3 View
Other histological stains (e.g., methyl green) aside, which would be a better counterstain for nickel-enhanced DAB immunohsitochemistry: cresyl violet or neutral red? Should I alter my staining...
01 January 2015 1,017 3 View
