Vincent J. Coppola

6 Questions 4 Answers 0 Followers

Questions related from Vincent J. Coppola

Does tissue need to be perfused for congo red staining?

Does tissue need to be perfused for congo red staining, or can is be done on non-perfused (i.e., bloody tissue)? Also, is 10% formaldehyde in PBS an appropriate fixative?

03 March 2019 6,250 1 View

Best way to visualize diagonal band in avian brain?

I would like to apply a stain that will really make the diagonal band in the pigeon brain "pop-out". I can identify it in cresyl violet-stained sections, but sometimes it is quite difficult to...

04 April 2015 1,490 3 View

Can anyone provide some insight with the following BrdU concerns?

I have recently begun working with BrdU to label proliferating cells in the VZ of pigeons (thus, this is a 24 hr survival) -- the quality of the IHC has been less than impressive, but that's...

03 March 2015 7,678 1 View

Is there any way to salvage tissue after a bad perfusion?

Everything looked good until I exposed the skull and found that the brain was bloody. I planned to do DAB-IHC for BrdU in the ventricular zone. Is there anything I can do pre- or post-cutting to...

03 March 2015 1,351 7 View

Is anyone familiar with heating a Corning 24-well tissue culture plate?

Does anyone know if a Corning 24-well tissue culture plate can be heated to 37-degrees C (98.6-degrees F) without any issues? The last thing I want is the plate to melt, or some other catastrophic...

02 February 2015 8,033 3 View

Which would be a better counterstain for NiDAB IHC: cresyl violet or neutral red?

Other histological stains (e.g., methyl green) aside, which would be a better counterstain for nickel-enhanced DAB immunohsitochemistry: cresyl violet or neutral red? Should I alter my staining...

01 January 2015 1,037 3 View