I am performing stereotaxic injection in mice. In the Paxinos Mouse Brain Atlas it appears that the depth is relative to the brain surface at bregma (z=0). The hole I drill in the skull is tiny, so I cannot see the surface of the brain. I want to account for the distance between the skull surface and then add this value to my depth. This would include skull thickness, dura, and subarachnoid space, etc. Does anyone know what a typical value is? How much does it vary between mice and at different locations within mice? I'm injecting roughly 5mm rostral to bregma and 1mm lateral from the midline.
If you use the mouse brain atlas ( Paxinos, George, and Keith B.J. Franklin. The mouse brain in stereotaxic coordinates: hard cover edition. Access Online via Elsevier, 2001 ) to get your coordinates the 0 value is the skull level at bregma, not brain surface as you state in your question. So you actually do not need to know what the distance is between skull level and brain surface is.
However, judging from my experience and the brain atlas I would say it is approximately 700 um. Take a look at this image from the brain atlas
http://labs.gaidi.ca/mouse-brain-atlas/
look at the coronal section at 0.02mm from bregma. As you can see the 0 point in the dorsal ventral axis is above the brain surface.
good luck
Nils
Surface of the dura is good. Your hole should be big enough to see the dura (drill carefully down to it), and you should then make a small incision in it, not just punch through.
If you use the mouse brain atlas ( Paxinos, George, and Keith B.J. Franklin. The mouse brain in stereotaxic coordinates: hard cover edition. Access Online via Elsevier, 2001 ) to get your coordinates the 0 value is the skull level at bregma, not brain surface as you state in your question. So you actually do not need to know what the distance is between skull level and brain surface is.
However, judging from my experience and the brain atlas I would say it is approximately 700 um. Take a look at this image from the brain atlas
http://labs.gaidi.ca/mouse-brain-atlas/
look at the coronal section at 0.02mm from bregma. As you can see the 0 point in the dorsal ventral axis is above the brain surface.
good luck
Nils
Thanks Nills. 0 value at skull bregma makes sense. I was not accounting for the fact that the skull level actually briefly elevates (more dorsal) as you move lateral from the midline, so I was thrown off by this sagittal image (lateral 1.08) where the brain at bregma equals zero.
http://labs.gaidi.ca/mouse-brain-atlas/?ml=1.08&ap=-2&dv=3.2
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