Best way to remove PTFE transfer film from counter surfaces (stainless steel and glass) for wear testing (pin-on-disk and tribometer spherical tips)?

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Loss of reactant in synthesis involving Amberlyst-15?

I am currently trying on a synthesis involving Amberlyst-15 (polystyrene with sulfonic group at p-position) as a proton donor. When following the reaction process by quantitative 1H NMR, I am...

20 October 2021 6,387 4 View

When 345 and 500kV transmission tower configurations are paired with different conductors (finch, parakeet, etc). how does this affect a power system?

In other words how do conductors with different resistor, capacitor, and inductor ratings perform in a power system when matched with different transmission tower configurations?

02 March 2018 3,215 3 View

Does anyone have experience with double data entry in REDCAP?

I've used REDCAP for several years but we're initiating data collection on a new project that includes 10 parent survey instruments and 13 forms that research assistants are entering standardized...

07 August 2017 7,279 3 View

How can I do a double size selection in order to obtain fragment between 1.5kb and 2.0 kb (no shearing)?

Using AMPure XP Beads if possible. Do you have any protocol or idea of the beads proportion I have to use ? Thanks

16 September 2015 624 3 View

GAUSSIAN IRC - how to change the value of RMS gradient Norm

Dear All Im studing reactions by DFT with gaussian (9 instead of 16). Currently im performing IRC on my TS# . For the full serie, all of them show a interesting shape in the reverse path 1-...

01 January 1970 2,467 2 View

Bio-clinical viral samples conservation ?

I'm currently studying virome and viral genome diversity in clinical samples. Can you suggest me references describing optimal conservation for clinical samples in a hospital laboratory?...

01 January 1970 9,914 3 View

How to learn more about SPSS and its Application?

I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.

11 August 2024 9,101 4 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

Text-Communication from the M1 Hand Area using BCI—and then there is Elon Musk?

Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...

10 August 2024 7,180 0 View

Has anyone applied Python in the field of textile engineering for data analysis, automation, or smart textiles?

I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...

10 August 2024 7,429 2 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

How are iso-frequency contours plotted?

Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...

07 August 2024 1,937 1 View

How to prepare the nanoparticle treated fungal sample for Environmental SEM analysis?

A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.

07 August 2024 5,307 1 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

Anand Pai

PTFE owing to its excellent chemical resistance, normally goes into solution near its crystalline melting point (~327oC). The removal may be possible under the action of a strong base metal ( like Na in molten state), fluorinating agents, imines and amines.

Dr Thoguluva Raghavan Vijayaram

By acid pickling.