Best purification tag for Saccharomyces expression?

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Which cointegration approach should I use when the variables are integrated in different orders?

I am working on a 21 sample size data time series, and after applying ADF test one of my variables are integrated of order 2 I(2), two variables are integrated of order 1 I(1) and two are...

08 June 2024 5,787 12 View

Can you give me an introduction about my research?

Cultivating a Love for Reading Amongst 21st Century Learners through Think, Pair and Share.

23 April 2024 4,916 0 View

If the curvature of spacetime at the beginning of the universe is infinite, how can the universe evolve to now in a finite amount of time?

According to the Big Bang theory, our universe began with a singularity, where spatiotemporal curvature is infinite. That means it would take infinite time to come to the present. That's...

19 March 2024 9,482 2 View

What should be the color of my solution after rotary evaporator?

Hello, I am currently doing a research about essential oil extracted using soxhlet extraction from guava (Psidium guajava L.) and my solvent is n-hexane. I am just wondering what should be the...

12 March 2024 4,525 4 View

What Should I Put on an XRF Table when Presenting a Paper?

I conducted an XRF test for my research and have a raw data file. However, I noticed that some of the chemical compounds such as K2O, Na2O, Fe2O3, etc. are missing, while their elemental...

02 March 2024 2,756 3 View

High voltage line noise and livestock?

I'm looking for research on the impacts of high voltage line noise on livestock. I tried the webpage search bar, but I keep getting pushed to a "are you a human" test and failing, sometimes even...

27 February 2024 4,395 1 View

What is the correct way to manage participants in a split head study?

Hello, My Masters research is a systematic review and meta analysis. One thing I am struggling with is when a study has undertaken a split head comparison (intervention on one side of the...

20 February 2024 257 1 View

Why does most laboratories still use gel separatory serum tubes over lithium heparin gel tubes?

The journal paper "The transition from gel separatory serum tubes to lithium heparin tubes in the clinical laboratory" discusses the advantages of lithium heparin over the traditional gel...

06 February 2024 7,581 1 View

Is it possible to remove multiple papers easily?

ResearchGate "found" lots of papers on which I'm not the author but share the same name with the actual author. There are over 100 of these! Is there a simple way to remove multiple papers or do I...

11 January 2024 6,531 2 View

What are good GPS trackers for recording the daily grazing movements of livestock?

I am looking for recommendations for GPS trackers to record the daily grazing movements of goats, cattle, and camels in the Mountains of Dhofar, Oman. In the past, I have used a tracker from...

18 December 2023 4,577 4 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

What is the best blank for nanodrop if I want to read a recombinant protein concentration?

Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC

01 August 2024 967 3 View

What is the relationship between protein structure and N or C terminal tagging choosing?

I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...

28 July 2024 366 3 View

How does the Retention time effect the Biogas Production?

List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?

27 July 2024 4,800 4 View

Problems with ion exchange chromatography?

I'm having problems with ion exchange chromatography. After applying my sample (a fluorescent protein), a preferential path inevitably forms. The matrix I use is DEAE-Sephacel. Could someone...

27 July 2024 6,605 0 View

Can anyone help me with in providing the procedure for purification of GSTtagged protein by gst affinity chromatography without an automated machine?

Purification of gst tagged protein

25 July 2024 1,720 0 View

How do regenerate Amberlite XAD 4 resin for further cycles of use for removing 0.5% Sarkosyl from recombinant protein solutions?

I use 0.5% sarkosyl to solubilise recombinant proteins contained in Inclusion bodies of E. coli. sarkosyl very effeciently solubilizes proteins from Inclusion bodies and I am trying to use...

25 July 2024 5,001 2 View

Why my IgG antibody looks fragmented on non-reducing SDS-PAGE?

I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...

23 July 2024 6,664 6 View

Dear fellow researchers studying in molecular biology, are there any tips on how to avoid plasmid dimerization when extracting plasmids?

Using INTRON's DNA-spin Plasmid DNA purification kit to extract plasmids from specific E.Coli strains, my team and I have encountered numerous plasmid dimerizations that lead to over-weighted...

23 July 2024 3,490 6 View

J. Stolz

which type of protein are you expressing? Can you provide more information?

Maurice Ekpenyong

You'll never know except you try. Please take a look at this document. Article Expression and Purification of Recombinant Saccharomyces cer...

Mark A Arbing

I should have said that it's soluble cyoplasmic proteins - a number of different COVID related targets. I'm not a GST fan, because it dimerizes, so that's not an option.