Hello, I want to order Midiprep plasmid isolation kit, but I can't decide which brand to use. Can you recommend the brands you always use and think are highly effective?
Dear Moein Iranmanesh
there are a lot of good midiprep kit provided from seveal companies.
In the past i tested a lot of miniprep provided from several companies (eg. Qiagen. Omegabiotek, thermo, mc
Actually the one the i like a lot is
https://www.thermofisher.com/order/catalog/product/A35892
sinci it not only guarantee very good performances but it has very simple protocoll that do not require high speed centrifugation for the removal or cell debries and for washing after ethanol or isopropanol precipitation but it allow to remove cell debries with a simple filtration and to purifity the DNA in the coloumn with out any additional step.
best
Manuele
if you have not already ordered a kit yet, i suggest you order the midi kit from Zymo Research. I used the manifold vacuum to finish the whole process without the use of centrifuge. The whole process is easy and timesaving, with a good amount of plasmid yield.
Hi Moein,
I completely agree with Jianfeng Lin about the zymo kit! I use their kits ( midi, maxi) since 2 years and they're great. If you use the manifold vacuum you can do the prep in less than 20min.
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