Can I get to know the experimental design for water expose gene expression study using human cell lines?

I have planned to do cell based gene expression (human) study using disease suspected water sample. For that, I planned to use human cell lines. Still I don't have plan regarding water sample, how...

08 March 2018 3,837 3 View

Can anyone advise me about water storage condition for genetic study?

We planned to do gene expression study using disease suspected water sample.

10 September 2017 4,930 8 View

Can we use post-mortem peripheral blood for disease related gene expression analysis study?

03 September 2017 7,170 1 View

How and why at pH 8 gives more efficiency on removal of Cr heavy metal?

I have done my soil washing using EDTA and got result at pH 8 gives more removal of Cr.What is the reason for base pH?usually removes for acid pH but for Cr gave more efficiency at pH 8..

14 June 2016 1,425 4 View

What is the permissible limit of heavy metals in soil?

please tell any IS standards for permissible limit of heavy metals in soil?

06 May 2016 9,681 18 View

How to maintain pH in the nature of base?

I want to maintain pH in base but also it should be in the nature of acid. My solution to test in ICP-AES for heavy metals is EDTA washed soil solution which is base nature. To keep in ICP, it...

09 April 2016 2,160 9 View

Fertilizer recommendation/Time of application for sorghum crop in pots?

With condition one plant per pot and 30days growth period to ensure uniform growth.

05 January 2016 1,360 4 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

Differences between sheep blood with Alsever solution vs citrated/defibrinated sheep blood in microbiological media?

I am aware of a study that has compared the use of citrated and defibrinated sheep's blood in microbiological media (Hair Sheep Blood, Citrated or Defibrinated, Fulfills All Req... ), but can...

10 February 2021 5,927 1 View

Hi everyone, I want to lower the pH of microbiological broth, what are the best options?

I will be using the lowered pH broth for MIC test of some disinfectants, and I do not want the acid to have any interfernece with MIC results.

03 February 2021 6,959 4 View

How do microbes tell the presence of an inorganic minaral in the soil?

Due to their ubiquitous nature, there is a tendency of the presence of microbes in a given mineral(s). For instance, if microorganisms that love the of pH less than 6 in the pH value, is isolated...

03 February 2021 4,630 10 View

How do you quantify bacterial qPCR data?

Hi all, How would you use qPCR to quantify the amount of a particular bacterial species in a sample experimentally? I understand you could use specific primers for the 16s V region of a...

01 February 2021 7,153 4 View

How can I know the stationary phase of growth of several bacteria?

How can I know the stationary phase of growth of several bacteria? We are aiming to collect exoproteome of several bacteria. We know that the ideal time to collect exoproteome is during the...

31 January 2021 4,528 10 View