I am trying to identify a kit to assay glucocorticoid receptors (GRs) bound to GREs in a select brain region. I have an antibody to perform the immunoprecipitation part of the assay, but I am trying to identify a kit. This is a kit that seems to work for some people.
https://www.epigentek.com/catalog/epiquik-chromatin-immunoprecipitation-chip-kit-p-59.html
Any suggestions?
Hi Dayan,
For your brain tissues, instead of the P-2002 EpiQuik ChIP kit, which is ideal for cells as starting material, I would recommend the P-2003 EpiQuik ChIP kit that is specific for tissue input samples and is also highly cited. Please refer to the following articles describing the use of brain tissues with the P-2003 kit:
Article A Double-Edged Sword Role for Ubiquitin-Proteasome System in...
https://academic.oup.com/ijnp/article/12/1/73/627497
Okay thanks much Michael!!!! I tried the p-2002 kit and will post the results here (hope I remember to do this). If it does not work, back to the drawing board and will try to p-2003 kit.
- Badges
- Science topic
- Similar topics
- Microbiology
- Assays