Dayan Knox

7 Questions 29 Answers 0 Followers

Questions related from Dayan Knox

Best chromatin immunoprecipitaiton kit for brain tissue?

I am trying to identify a kit to assay glucocorticoid receptors (GRs) bound to GREs in a select brain region. I have an antibody to perform the immunoprecipitation part of the assay, but I am...

12 December 2019 2,799 2 View

Anyone aware of good mouse c-Fos and c-Jun primary antibodies?

So after being burned by the antibody roulette I decided that I should probably ask someone on this forum for some antibody help. I am starting to do double labeling of neurons using Alexa Flour...

10 October 2017 9,918 1 View

What's a good protocol for using Alexa Flour antibodies for IHC in brain tissue?

So I typically use western blot and IHC (near infrared imaging in both) to assay proteins in brain tissue. Sometimes I used DAB reactions as well. However, I've had the need to switch to Alexa...

08 August 2016 7,988 6 View

Does anyone have any experience using the iBind Western system?

So life claims this new system will greatly improve westerns. Here is a link to the system (below). Is this true? Anyone has experience with this.

01 January 2015 3,727 5 View

Just curious what everyone thought about the initiative from the NIMH referred to as Research Domain Criteria (RD0C)?

For those of you who don't know about it, you can find information here:...

05 May 2013 5,600 3 View

For Western Blots, should I transfer proteins using standard chamber or switch to a semi-dry transfer method?

So I was wondering what is the best method for transferring proteins from gels onto nitrocellulose or pvdf membranes. Semi dry or standard chamber? I have only ever used standard chambers, but was...

12 December 2012 5,322 34 View

Pros and cons of using the Li-cor scanner vs. standard chemiluminescence for Western Blot electrophoresis

Does anyone have experiences with both the Li-cor Odyssey Scanner system, which uses flurorochrome

04 April 2012 5,985 39 View