If you have a microscope and hemocytometer in the field, suggest you use Randolph’s Stain to perform absolute eos count. It’s Phloxine B and Methylene Blue dyes. Eosinophils stain a majenta/purple color. Don’t recall the formula at the moment.

Thank you Steven,

I will look into Randolph's Stain :-)

Cheers

Which type of sample do you want to stain? For mouse bronchoalveolar lavage samples we successfully used Diff Quick (which is H&E based). For tissue samples (i.e. lung cuts), one can detect esinophils on H&E stained slides (not always easy, but works if the staining is done nicely) or with LUNA staining. There are some good papers out about other eosinophil-specific staining protocols (i.e. PMC2664312).

I will be looking at blood drawn from Crocodiles, and I need to look for Eosinophils as well as parasites in the blood (just to investigate presence)

Read this paper: Improved Randolph Stain for Direct Leukocyte Differentiation and Determination of Total Eosinophil Count in a Hemocytometer.

Best regards,

Tatiana

Thanks Tatiana,

I'll have alook straigh away