Why reinvent the wheel? Cell Biolabs has a 4-HNE lipid oxidation ELISA lit.

Thank you Bruce.

Depends what you want to do and the sample type. If you want to measure unreacted HNE, mass spec is the best way. However, HNE can be rapidly metabolized to GSH adducts or to the acid or alcohol. Most kits are not selective for HNE, however, and may react with other unsaturated aldehydes.

If you want protein adducts, antibodies will work.

Thank you Matthew!

This kit from Cell biolabs is vastly expensive, any cheaper ways to assess 4-HNE?

Hello, I'll renew the topic I'm looking for a fluorescent HNE test. Does it exist?