Presence of protein in a cell line that is used as a negative control for that specific protein?

I have quite an absurd issue. for the information: 1) I have a cell line (K), that i have seen in literature being used as a negative control for my protein (P). However, for me the cell line...

30 May 2019 3,213 3 View

Different genotyping result for the same sample under same conditions?

27 February 2019 331 13 View

Total RNA stability at room temperature (+25 C)?

Hello everyone, I'm facing a problem regarding my pure extracted RNA samples, I extracted them from tissues of rats' livers and kidneys immersed in RNAlater, using the promega SV isolation...

04 November 2017 3,737 5 View

Quality costs in different phases of slip casting?

05 October 2016 5,317 3 View

Comparing different qPCR amplicons, how to account for different length of amplicons when using SyberGreen?

Dear all, I performed several qPCR, which also worked fine, however, now during the evaluation I encountered an issue. My problem is that due to the restriction when choosing primers, I ended up...

05 August 2014 3,406 2 View

For ELISA, which capture/coating AB concentration should I use?

I am developping an ELISA, which seems to work, although I am puzzled by the titration of the capture AB. The Assay: All samples were in dublicates, volume used 50ul, except 150 for blocking,...

08 April 2014 6,566 3 View

Is there any evidence that links bacteria with oral cancer?

Certain microbes have been linked with oral cancer like HPV and syphilis. Recent research suggests that anaerobic bacteria may be linked with colon cancer.

26 August 2013 698 24 View

I focus for a training course

I'm a PhD student and I'm working on "Invasive Alien Plants in Tunisia: invasiveness and management strategies." I hope to have the opportunity to benefit from a training period in a laboratory...

17 December 2012 8,704 2 View

Can we modify the pre designed Deep networks like googlenet and alexnet to use them for signal classification?

Signal classification using Deep Learning

01 January 1970 9,370 4 View

What affects will the internet of things (IoT) have on infrastructure and smart cities region?

Is the competencies of the clever grid, smart homes, and its combined with IoT components in other public utilities, including roadways, sewage, and water transport can make a contribution to...

01 January 1970 7,751 5 View

Why should we choose deep learning to machine learning? Why is CNN better for image classification? Can I get Typical Answer?

What Characteristics makes CNN work better?

03 March 2021 1,458 4 View

What are some of the research gaps in machine learning and artificial intelligence in africa?

i would to know some of the research gaps in the artificial intelligence field in most african countries.

03 March 2021 6,145 3 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Can you share me how teacher education is managed in your respective national educational system?

I feel that the practice in teacher education in my country is below the expected performance level due to very poor management system. Hope I will learn something from your experiences.

02 March 2021 1,516 4 View

Can NFL theorem be valid in infinite search space in ML?

NFL theorem is valid for algorithms training in fixed training set. However, the general characteristic of algorithms in expanded or open dataset has not been proved yet. Could you show your...

01 March 2021 1,189 3 View

What is the difference between L1 and L2 regularization in machine learning?

L1 and L2 regularization

28 February 2021 4,187 3 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View

Is there any Machine Learning/AI algorithm that lets you predict with incomplete features without missing value imputation process?

For instance, the model is trained with A,B,C,D,E features to predict F target. Then, I want to make prediction on a new data, but with only A,C,D,E is known without doing missing value imputation...

28 February 2021 1,556 6 View

How can I represent SDS-PAGE results for machine learning analysis?

I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...

27 February 2021 9,176 3 View