The Chlorella algae is grown heterotrophically. When it is harvested it is put into a cooling tank, but here they turn brown in a week. What is the exact cause of this? and what may be a solution to prevent them from getting brown?
you may store chlorella in an alkaline media (pH- 9-10). In acidic media (
I want to quantify autophagic flux in a ipsc-cardiomyocytes. Therefor, I was wondering whether someone had used and/or purchased a ipsc line with a GFP-LC3 reporter? Thank you
14 May 2023 4,826 0 View
We are looking for the Parent-Child Interaction Questionnaire-Revised [PACHIQ-R] (Lange et al., 2002) translated to Arabic, Persian/Farsi and Tigrinya for a research project assessing an...
28 March 2023 1,292 0 View
We are looking for the translations of the Parental Stress Scale (Berry & Jones, 1995) in Arabic, Persion/Farsi and Tigrinya for a research project with refugee parents in The Netherlands.
28 March 2023 1,013 0 View
The Public administration reform that Libya under went after independence.
14 March 2023 10,033 1 View
I nucleofected iPSCs from one patient line, grew colonies and attempted to genotype them. i Had previously run a PCR gradient to determine the best Tm for the primers. most of the PCR products...
05 October 2022 5,799 0 View
We have found a potential patient disease causing point mutation in an exon of a gene. We are interested in the possible differences between the expression of single nucleotide variation between...
19 January 2022 7,293 3 View
Hi, I wanted to analyse my longitudinal data (2 time points) with a simple change score in a regression, however, I came up with 3 different models and I am not sure which one to use or why. I...
17 August 2018 5,208 3 View
I've run IHC (using EM48 as primary; ABC kit; visualized with DAB; Nissl counterstain) on mouse brain sections (striatum). How do I count mutant huntingtin inclusions in these sections using ImageJ?
01 April 2018 8,506 1 View
Hi, I'm just starting to look at analysing my longitudinal data with mixed models in SPSS. For each time separately I analyse my data with partial correlations, hierarchical regressions and...
19 March 2018 2,816 4 View
We will be lysing mouse brain tissue for the co-IP of TLR4 and MyD88, followed by a western blot.
23 October 2017 7,805 2 View
I am working on microalgae cultivation using waste water. The initial concentration of nutrients were less but the microalgae has achieved biomass growth of 2 g/L. The final concentration of...
08 August 2024 4,812 2 View
i am comparing the growth performances of filamentous and non filamentous forms. in a case where I can not count the cells of the filamentous algae using a hemocytometer, is it okay to do a...
30 July 2024 5,985 2 View
I am attempting to isolate picocyanobacteria from seawater (pre-filtered with a GF/D membrane) using the pour plate technique and filter plating method (Kearney et al., 2022) on Pro99 and SNAX...
30 July 2024 1,040 1 View
Study case : turbo-generator cooling I have a small rotor-stator gap with slots on the stator. The rotor is rotating at high RPM (around 10,000 RPM). The cooling fluid is also circulating...
10 June 2024 579 0 View
Hello, community, Could you please clarify whether current legislation permits the reuse of algae biomass after it has been used to treat non-hazardous decontaminated laboratory organic waste?...
05 June 2024 5,040 1 View
I am trying to design a heat removal system for a specific application. As the heat flux is very high, the only way to reduce the temperature is by increasing fluid(water) velocity. I am worried...
27 May 2024 3,804 4 View
Hi everyone, I'm having trouble with my hydroponic cultures for Arabidopsis thaliana, since lately I've started having algae contamination on them. I have tried using several sterilization...
21 May 2024 4,840 1 View
Can heating degree days be negative and how do you calculate cooling degree-days in Celsius and heating time and what is heating and cooling time constant?
16 May 2024 8,300 6 View
Hi TRNSYS users, I am new into TRNSYS and would like to model a smart system that can extracts energy from the output of a cooling mode heat pump and use it in either of the following ways: During...
16 May 2024 5,052 0 View
Hey there. I'm a Science, Business and Innovation student and for my thesis project I'm currently doing research on different production methods for large-scale cultivation of spirulina....
13 May 2024 368 1 View