Because Ca2+ in milk interferes with Mg2+ (necessary for Taq function), how I can resolve this problem in my PCR reaction? My sample is milk.

05 May 2013 1 8K Report

Please guide me.

Try adding EGTA (ethylene glycol tetraacetic acid) to your PCR mixure. The EGTA will scavenge Ca2+ ions but not Mg2+ ions.

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