How the Ground Plane implantation is performed without damaging (and w/o varying the undoped nature) the top Si (8-10 nm) in FDSOI technology ?

It is a general practice to introduce Ground Plane in FDSOI devices under the BoX. The Ground Planes are introduced by Ion Implantation through Top Si layer and Buried Oxide. I wanted to know that...

24 February 2021 5,974 4 View

How can I maintain a surface at isothermal condition, say at 300 deg Celsius.?

I have 6 heaters each of power 0.5 kW and mounted below a curved mild steel sheet of thickness 4 mm. The heaters and the below section is covered and insulated. I want the top surface to be...

05 November 2020 199 5 View

What is the time required for an enzyme or an protein molecule to consume an ATP molecule during a reaction?

In what time do a protein body consumes an ATP molecule for an active process to happen?

02 October 2020 8,135 4 View

Person Temperature detection using thermal images taken from thermal camera?

I have tried based on pixel value with a referential ground-truth temperature of some different pixel value, a region of interest (thermal image of a person's forehead ). I am getting near about...

15 July 2020 336 1 View

How to calculate my research impact factor including first and second authorship?

If I have published x no of papers as a first author and y no of papers as the second author, how can I calculate my research impact factor not considering the citation? Thank you in advance

30 May 2020 5,086 17 View

Can anyone suggest how to design a transistor model in ansys circuit?

Can anyone suggest how to design a transistor model in ansys circuit. snp file with respect to different bias is available but from the datasheet I want to design transistor and observe it's...

05 March 2020 2,582 3 View

What is the electrical conductivity and relative magnetic permeability of Steel P92 material ?

What is the electrical conductivity and relative magnetic permeability of Steel P92 material ?

25 February 2020 8,433 2 View

Is it possible to track endoplasmic reticulum and calcium present in that simultaneously by using dye? Do there exist any other alternative way ?

Is it possible to track endoplasmic reticulum and calcium present in that simultaneously by using dye? Do there exist any other alternative way ?

12 February 2020 9,022 3 View

How can we analyze cancer markers proteins in Co culture assay (PBMCs and Cancer cell)using WESTERN BLOTTING?

We are checking cancer cell death by activated PBMCs in a co-culture system. We specifically want to analyze the markers (Caspases, PI3K/mTor/AKT, BAX BCL2 and several other markers) using...

30 January 2020 7,697 2 View

How to correctly simulate deposition which shows induced defects and dislocations (if any due to deposition) in Silvaco ATHENA TCAD tool ?

I am trying to simulate the deposition of Silicon over SiO2. In reality, when we try to deposit a crystalline Silicon over SiO2 (amorphous), we get rather a polycrystalline Silicon. In ATHENA...

22 November 2019 4,916 4 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

Universal bacterial primers targeting a mRNA?

Hi, I'm looking for universal primers targeting a bacterial mRNA to quatify bacterial load by qPCR in a complexe mixture (eukaryote and prokaryote RNA). Ribosomal RNA being depleted, I absolutly...

22 February 2021 9,969 3 View

Does anyone have any ideas why drug-resistant malaria is increasing in Southeast Asia even when those drugs are no longer used?

When dealing with bacterial toxins, there are three varieties of microbes that develop in your petri dish: toxic, resistant, and susceptible. Initially, there are only toxic and vulnerable...

21 February 2021 6,084 5 View

Could you please share your experience with me on the type of 96 wells plate for MIC determination: Treated Vs Untreated and Round-Vs Flat-bottomed?

I am working on the MIC determination of various antibacterial agents (both water-soluble and- insoluble) against S. aureus, MRSA, and S. pyogenes. Some guidelines recommend using round-bottomed...

17 February 2021 7,029 3 View

What are the best cations for preparing competent cells, Ca2+ or Mg2+?

I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...

16 February 2021 8,519 5 View

How much amount of DNA (ligation mix) should I use for transformation?

Hi Fellow Scientists! I am trying to clone my 300 bp insert into a 6 kb vector. I run a few different ligation reactions using (1:3 vector: insert molar ratios) 10, 20,30, and 40 ng/ul vector...

15 February 2021 9,563 6 View

Would using an asRNA for repressing transcription of a high expression Listeria monocytogenes non-coding RNA harbored in a plasmid be a viable option?

Hello all, I am interested in deleting a highly expressed non-coding RNA located on a Listeria monocytogenes plasmid. Currently, there are not any optimized gene deletion methods for Listeria...

09 February 2021 4,994 1 View

Does DNA A-tailing survive after agarose gel electrophoresis and purification?

Hi everyone, I need to clone in pGEM-T vector the result of amplifying a metagenomic library by PCR with a high fidelity polymerase but I only want to clone the fragments of an specific size...

04 February 2021 6,051 8 View

Multiple insertions with Q5 Site-directed mutagenesis?

03 February 2021 221 2 View

What is the best bioinformatic tool to predict the interaction of peptide with bacterial cell wall membrane?

Hello everyone, How are you guys doing? I'm currently working with antimicrobial peptide (AMP) project and one of our objectives is to predict the interaction of peptide against bacterial cell...

01 February 2021 1,351 3 View